Description
Fragmenting genomic DNA (gDNA) is key to improving long-read sequencing performance and data yield. NEBNext UltraShear® Long Read is a fast, flexible enzymatic method for gDNA fragmentation, designed for use upstream of Oxford Nanopore Technologies® (ONT) and PacBio® library preparation and sequencing. This time-tunable solution allows precise fragment sizing from 2 kb to 30 kb in as little as 30 minutes, while preserving native methylation marks. It works with a broad input range (250 ng–5 µg) and is compatible with automation, making it easy to scale as sequencing needs grow. NEBNext UltraShear Long Read provides a cost-effective and reliable alternative to mechanical shearing methods.
- Improve your sequencing yield while maintaining high N50 values
- Achieve unbiased fragmentation from 2–30 kb for diverse long-read applications
- Preserve native methylation for downstream epigenetic analyses
- Scale effortlessly with an automation-ready process and flexible input range (250 ng–5 µg)
- Cut costs and simplify workflows by eliminating specialized instruments and consumables
